The ants I killed probably had a biomass equal to a small forest, had no external food supply - as the two corridors out of the queen chamber were both battle sites with nothing getting past. Realism has already long departed this situation.
It would make sense to wage an endless war with the ants if you tried to stay away from the nest, as you would be allowing the a fairly large amount of time to grow. Same total population, but slower numbers regeneration. But if you manage to make it inside, it really shouldn’t be quite this ridiculous to clear the final room. Difficult sure, but not like this.
And if you really want there to be more ants, make the nest bigger, with a higher ant/room count, but don’t cause them to spawn at a rate that defies all logic. There are plenty of ways to handle this without making an unbeatable room at the end.
I mean, it’s not even the numbers that bother me; not really. If there were a couple hundred guarding the queen chamber, but those numbers were static - or regrew at a more reasonable rate, like a couple dozen a day - then it would be fine, and feel like a worthwhile challenge. Like after wading through the ant hordes you have finally reached the end, and kill the queen. Instead it was a pointless grind where progress was impossible, absent console commands, or a LOT of explosives.